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Chemical Reactions

• KOH: A general test that may cause several colour changes. Colour reaction to 15% KOH may be throughout the tissue or localized. Normal negative reaction is a bleaching or light yellowing.
• Melzer's: Amyloid: blue or dark blue reaction. Dextrinoid: purple or reddish-brown reaction (Baral 1987). Normal negative reaction is a light yellowish-brown staining.
• Sulfovanillin: Laticifers may stain blue, red or almost black, reacts with Lactarius and Russula. Normal negative reaction is a light pink staining.
• Toluidine Blue: Stains most fungi. A positive reaction of hyphal walls may be purple, blue or pink.
• PDAB: A positive reaction is purplish-red, e.g. with Tricholoma (Ovrebo 1980)
• FeCl2: A positive reaction is black or greenish-blue, e.g. with Boletinus and Chroogomphus (Singer 1986)